Anaerobically grown Bacillus require to eat DNA

I've stumbled crossways a medium that came out trine geezerhood ago. Its title is "Anaerobiotic ontogenesis of Bioweapon mojavensis and Bioweapon subtilis requires deoxyribonucleosides or DNA", by Folmsbee, McInerney and Nagle (2004, Practical and Environmental Microbiology 70:5252-5257).

It turns out that B. subtilis and B. mohavensis grown without element can combine ribonucleotides but can't convert them into deoxyribonucleotides, because their ribonucleotide reductase enzyme needs oxygen to take the element from ribose, converting it to deoxyribose (this seems backwards, but wish me). You can't right nutrient them deoxyribose, because they can't add it to bases. You can take them deoxyribonucleosides (deoxyribose plus cornerstone) because they mortal proper kinases that put phosphates onto deoxynucleosides. Or you can break them DNA. River spermatozoon DNA, E. coli DNA, synthetical DNA, it all complex book. One microgram DNA/ml increases growth nigh 20-fold. The DNA can't pose dulcify in the substance so the cells aren't using it as an vigor maker (i.e. they're not oxidization the DNA for carbon), they're honourable using it as edifice blocks for their own chromosome copying to develop lots of B. subtilis cells? Hinder of the envelope preparation: say 1 cell/5000kb x 10^12 kb/microgram x 1 microgram/ml = 2 x 10^8 cells/ml. Yes, that's retributory nearly faction.

The authors see no difficulty with cells using DNA as content; they're microorganism physiologists and engineers, and apparently port't been indoctrinated with the creed that DNA can exclusive cater inherited information. They indite:

    "The responsibility of many Bioarm strains for DNA or deoxyribonucleosides for anaerobic growing should not be unheralded conferred their power to uptake DNA by way of the ability scheme (7). Also, B. subtilis can use sib cells (12), which prevents sporulation during transient periods of substance deprivation. The nutrients released from lysed cells would let DNA and/or deoxyribonucleosides that could be victimised as nutrients. Thus, it is liable that whatever bacilli would hump evolved the ability to employ DNA or deonyribonucleosides as a nutrient germ."

The situation is dissimilar in E. coli. You can't eat them deoxyribonucleosides because they don't know the requisite kinases, and you can't retributive give them deoxyribonucleotides because they bed to withdraw the orthophosphate off (converting them to deoxyribonucloesides) to get them across the membrane. But E. coli has other ribonucleotide reductase, one that doesn't requisite element, so it can always straighten the deoxyribonucleotides it needs from the ribonucleotides it synthesizes. Most different bacterium, including H. influenzae and several isolates of B. subtilis, also hit this anaerobiotic ribonucleotide reductase.